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	<title>High Content Screening &#8211; Think Bensonium</title>
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	<description>Sight through thought</description>
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	<title>High Content Screening &#8211; Think Bensonium</title>
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		<title>Image J InCell Macros</title>
		<link>https://bensonium.com/product/image-j-incell-macros/</link>
		
		<dc:creator><![CDATA[Roderick Benson]]></dc:creator>
		<pubDate>Fri, 04 Sep 2020 19:10:25 +0000</pubDate>
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					<description><![CDATA[This macro file extends the open source image analysis software Image J so it can convert a directory of tiff images produced by a GE InCell analyser high Content screening machine into a single hyperstack for consistent image manipulation. The program was written in 2011 when Imagen Biotech first purchased an InCell analyser. These programs maybe less useful now as I assume that GE have continued to develop their software. However I make this program available just in case someone does find it helps them with High Content Screening image manipulation using a GE In Cell Analyser.

<a class="nounderline" target="_blank" href="https://bensonium.com/wp-content/SoftwareDownloads/InstructionManuals/ImageJMacrosInstructions.pdf" rel="noopener">View Image J Macros for InCell Instruction Manual</a>

[softwarelink id=1046]]]></description>
										<content:encoded><![CDATA[<p>The GE In Cell analyser produces separate tiff files for every field and channel captured by the instrument. In High Content Analysis users often what to pick example fields from a well which reflects a certain treatment condition of the experiment. Furthermore, a user may want to produce examples images from control and treated conditions to show a visual example of the dynamic range of a particular assay. Therefore, it is important that any pixel mapping used by image analysis software is consistently applied across all the example images.</p>
<p>The software produced by GE to deal with these challenges back in 2011 was limited. However, there is an open source software available called Image J which has very powerful image handling functions. It has been possible for us to write some extensions to Image J which allow users to load well images from an InCell Experiment and create a 3 dimensional hyperstack that allows one to quickly apply a consistent pixel map to all the images within the stack.</p>
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